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AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
零下20℃
- 保质期：
至少一年有效
- 英文名：
p66alpha
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
5µg
pLenti-GATAD2A-sgRNA (GATAD2A基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
GATAD2A基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | GATAD2A | 54815 | BC011684 | NM_017660 |
| About the gene | |
| Official Symbol | GATAD2A |
| Previous Symbol | - |
| Official Full Name | GATA zinc finger domain containing 2A |
| Synonyms | p66alpha |
| Location | 19p13.11 |
| Gene Type | protein-coding gene |
| Uniprot ID | Q86YP4 |
| Pathway/Library | Epigenetic Regulators Related Genes Library |
| Gene Summary | Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B. P66A_HUMAN,Q86YP4 |
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文献和实验Hu, R.; Qin, J.; Feng, W.; Song, X.; Huang, H.; Dai, C.; Zhang, B.; Chen, Y. Lysosomal zinc nanomodulation blocks macrophage pyroptosis for counteracting atherosclerosis progression. Sci. Adv. 2025, 11, eadu5271
Generation and Functional Analysis of Zinc Finger Nucleases
on homologous recombination, which can be stimulated by the creation of a targeted DNA double-strand break (DSB) near the site of the desired recombination event. Artificial nucleases containing zinc finger DNA-binding domains have provided important proofs
Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells
. These double-strand breaks can now be created in mammalian genomes by zinc finger nucleases (ZFNs). ZFNs are artificial proteins in which a zinc finger DNA-binding domain is fused to a nonspecific nuclease domain. This chapter describes how to identify
Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
editing using sequence‐specific nucleases. Sequence‐specific nucleases are composed of a domain that binds to the target gene via zinc‐finger or TAL effector‐based DNA recognition motifs. The DNA binding part is fused to the nonspecific nuclease domain
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