Sartorius赛多利斯1784C----------QM

Single Primer ("Semi-Random") P

Reamplification 40 µL H2O 10 µL 10x M-PCRB 10 µL 4 dNTPs @ 2 mM each 10 µL Pn primer @ 5 µM 10 µL Po primer @ 0.2 µM 10 µL DNA from part 1 10 µL "T/TS" @ 0.4 u Taq/µL --------- 100 µL Load glass capillaries and amplify: 30"x94°C 30

Preparation of Carboxypeptidase Y and Its Properties

is immersed in a water bath at 30℃ during kneading of the mixture. After liquefaction, add 600ml of distilled water, the mixture is stirred. The pH of the mixture is adjusted to 7.0 with 1 M NaOH under vigorous stirring, and the whole is kept at 25

Isolation of cell nuclei for the application in the cell-free system

- PROCEDURE Grow cells in a 160 cm2 flask (25 ml medium) up to about 75% cell density. Remove medium from the cell culture flask except 5 ml. To the remaining 5 ml add 25 µl of 4.2 mM Cytochalasin B (CB); Incubate 30 min at 37°C (cells