Sartorius赛多利斯1784B----------QM

Single Primer ("Semi-Random") P

a biased ratio of Po and Pn. Make 3 reactions with varying [Mg++]: 15 µL H2O 3 µL 10x H, M or L-PCRB 3 µL 4 dNTPs @ 2 mM each 3 µL Po @ 5 µM 3 µL template diluted to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL

Preparation of Carboxypeptidase Y and Its Properties

. ⑶ Activation: The precipitate is dissolved in 300ml of 0.05 M sodium acetate buffer, pH 5.0, and adjusted to pH 5.0 with 1 M acetic acid under stirring, then incubated at 25℃ for an additional 18～20h with a few drops of toluene added. ⑷

Isolation of cell nuclei for the application in the cell-free system

1 mM DTT 0.2 mM Spermine 0.5 mM Spermidine 0.1 mM PMSF 2 µg/ml Leupeptin 2 µg/ml Pepstatin 2 µg/ml Aprotinin 200 µl of 50 mM PIPES (pH 7.4), incl. 25 mM EGTA 80 µl of 1M KCl 20 µl of 1M NaCl 500 µl Glycerol 85 mg Sucrose 1 µl