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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 保修期:
两年
- 现货状态:
现货
- 供应商:
科邦兴业(北京)科技有限公司
- 规格:
盒
1784B----------QMinisartNYGF;0,20µm;25mm;nsterile;500pc
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文献和实验Single Primer ("Semi-Random") P
a biased ratio of Po and Pn. Make 3 reactions with varying [Mg++]: 15 µL H2O 3 µL 10x H, M or L-PCRB 3 µL 4 dNTPs @ 2 mM each 3 µL Po @ 5 µM 3 µL template diluted to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL
Preparation of Carboxypeptidase Y and Its Properties
. ⑶ Activation: The precipitate is dissolved in 300ml of 0.05 M sodium acetate buffer, pH 5.0, and adjusted to pH 5.0 with 1 M acetic acid under stirring, then incubated at 25℃ for an additional 18~20h with a few drops of toluene added. ⑷
Isolation of cell nuclei for the application in the cell-free system
1 mM DTT 0.2 mM Spermine 0.5 mM Spermidine 0.1 mM PMSF 2 µg/ml Leupeptin 2 µg/ml Pepstatin 2 µg/ml Aprotinin 200 µl of 50 mM PIPES (pH 7.4), incl. 25 mM EGTA 80 µl of 1M KCl 20 µl of 1M NaCl 500 µl Glycerol 85 mg Sucrose 1 µl
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