赛默飞世尔Thermo Fisher WASH BTL ML

v468 chapter 5 糖原合成酶激酶-3 的抑制

), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG

（共享）免疫组织化学染色FAQ

4 litres $104 ("Laboratory grade") Sigma 3 kg $42 (no purity details) Acros Organics (seems to be part of Fisher) sell three grades: 2.5 litres $24 (99%) 1 kg $23 (GC) 500 ml $36 (scintillation grade) If the 99% stuff is OK for histology

Inducing and Characterizing Liver Regeneration in Mice: Reliable Models, Essential “Readouts” and Critical Perspectives

1 Gauze pad 3 50‐ml conical tubes (e.g., BD Falcon) for holding solutions to wash the syringe