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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311340374
中文名称:
英文名称:X100 TYPE GF/D 2.4CM/100/PK
货期:热销品现货,其他请咨询




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文献和实验in the stir bar. 13.Bring the vlume up t 1 liter by adding mre f the Lysis buffer. 14.Add Tritn-X100 t a final cncentratin f 2% by vlume. Nte: The Tritn-X-100 will nt disslve immediately.It will g int slutin slwly ver night. 15.Stir gently ver night
Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh
Polyclonal and Monoclonal Antibodies
subcutaneously with 50 ug - 100 ug of Antigen in PBS and proceed with fusion 4 days later. OR III - Boost 3 days in a row with 15 ug Ag in PBS and proceed with fusion on 4th day. Cell Lines: 1) Myeloma; P3X63-Ag8.653. Origin: BALB/c, non
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