赛默飞世尔Thermo Fisher PLS STD NA

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

Northern Blotting方法(Howell Lab)

for at least 30 min before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml

Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH)

buffer (0.1 M Na2CO3 sodium carbonate, pH 9.0): To make 100 ml, weigh 1.06 g Na2CO3 and complete to 100 ml with DDW. Adjust pH to 9.0 by adding 10N NaOH and store at 4°C. 3. 2M TEAB stock: To make 500 ml, take 138.3 ml (101 g) TEA