赛默飞世尔Thermo Fisher ANTI-TOM1 A

Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells

, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial

免疫组化技术-Immunohistochemical Protocols

(mostly these are IgG's), and then an appropriate pre-conjugated anti-IgG molecule to detect the primary antibody. In addition to these conventional immunostainings, a newer method has been introduced with increased sensitivity. This is called the tyramide signal

Stripping Western Blots

per immunoprecipitation. You can use less (as little as 50) but the higher amount gives you a good, easily detectable amount of activity. 9.) To the lysate, add 1.5 μg of anti-ERK antibody (Santa Cruz, SC154 (Erk2) or SC93 (Erk1)), and incubate, with rocking