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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311836933
中文名称：
英文名称：X1000 COVER SLIPS 24X50 MM N°1,5
货期：热销品现货，其他请咨询
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文献和实验rpm at 4°C Prepare fluorochrome solution (see above) Cover each slide with 125 µl of the fluorochrome solution and a 24x60 mm2 cover slip. Incubate 20 min in a moist chamber at 37°C. Remove cover slips and wash slides 3 x 3 min at 45°
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
, and complete to 50 ml with DDW. Store at 4°C. Steps 1. Grow cells under standard conditions and seed onto gelatinized cover slips in a petri dish. Cells are grown to empirically-determined confluence such that they are sparse enough to facilitate automated
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