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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Vaccinia virus (VACV) (strain Copenhagen) L1R Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
蛋白构建A DNA sequence encoding the Vaccinia virus (VACV) (strain Copenhagen) L1R (P20540) (Ala3-Gly183) was expressed with a polyhistidine tag at the N-terminus.
表达宿主HEK293 Cells
种属VACV
预测 N 端Met
分子量The recombinant Vaccinia virus (VACV) (strain Copenhagen) L1R consists of 200 amino acids and predicts a molecular mass of 21.59 kDa. It migrates as an approximately 27.02 and 23.71 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验二硫键的形成)和NaCl[5]。 3、 供给丰富的培养基,创造最佳培养条件,如供氧、pH等。 包涵体的分离及溶解 对于生物制药工业来说,包涵体的形成也是有利的,不仅可获得高表达、高纯度的重组蛋白质,还可避免细胞水解酶对重组蛋白质的破坏。由于包涵体是蛋白质聚集而成的致密颗粒,分离的第一步是对培养收集的细胞进行破碎,比较有效的方法是高压匀浆结合溶菌酶处理,然后5000~20000g离心,可使大部分包涵体沉淀,与可溶性蛋白分离,接着,包涵体沉淀需用去污剂(Triton
间温度达到 50~60℃,抗体分子都能维持正确折叠,保持其应有的活性。(3)抗体和重组蛋白的纯度。Cambridge Bio 采用先进的抗体和重组蛋白纯化技术,确保抗体和重组蛋白产品的高纯度,保障其抗体和重组蛋白产品的稳定性。(4)抗体和重组蛋白的浓度。高浓度的抗体和重组蛋白产品可减少容器表面吸附造成的物质损失,高浓度的抗体和重组蛋白稳定性更好。Cambridge Bio 大部分抗体和重组蛋白浓度为 1mg/ml,抗体和重组蛋白纯度高,特异性好,效价更高。6. 重组蛋白的特性Cambridge
。 (2)抗体的高Tm值,在室温下,甚至短时间温度达到50~60℃,抗体分子都能维持正确折叠,保持其应有的活性。 (3)抗体和重组蛋白的纯度。Immune tech采用先进的抗体和重组蛋白纯化技术,确保抗体和重组蛋白产品的高纯度,保障其抗体和重组蛋白产品的稳定性。 (4)抗体和重组蛋白的浓度。高浓度的抗体和重组蛋白产品可减少容器表面吸附造成的物质损失,高浓度的抗体和重组蛋白稳定性更好。Immune tech大部分抗体和重组蛋白浓度为1mg/ml,抗体和重组蛋白纯度高,特异
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