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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70106-406-51
中文名称:
英文名称:SEAL ALUM TEARAWAY 20MM 100/PK
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文献和实验In Situ HYBRIDIZATION TO TISSUE SECTIONS
.C.T. (Tissue-Tek O.C.T. Compound) Paraffin (Paraplast Plus, Fisher) 8% Paraformaldehyde (Polysciences E.M. Grade 0380): Prepare fresh in 1X PBS. Dissolve 8g paraformaldehyde in 100ml buffer at 65-80 degrees C. Add 1-2 drops 50% KOH
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
, M. T. Cronin, C. P. Holmes and S. P. Fodor, Proc Natl Acad Sci U S A, 91, 5022-6 (1994). 19)S. P. Fodor, R. P. Rava, X. C. Huang, A. C. Pease, C. P. Holmes and C. L. Adams, Nature, 364, 555-6 (1993). 20)J. DeRisi, L. Penland, P. O. Brown, M. L
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