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- 2025年07月14日
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文献和实验Synthesis and Application of Highly Reactive Amino Linkers for Functional Oligonucleotides
50‐, 100‐, 200‐, 300‐, and 500‐mL round‐bottom flasks Condenser TLC, Merck silica gel 60F 254
Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
Coupling Peptides To Resin For Affinity Purification
checking the resin chemistry using a quick eyeball test. The amino resin will react with an NHS ester, whereas the original affigel and the iodoacetate will not. Take an aliquot (50ul) of resin at each step. Resuspend in 100 ul of buffer. Add 1 ul of 0.1 M
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