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文献和实验Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2
Phospholipases A2 (PLA2 ) are a family of ubiquitous lipolytic enzymes that are found both as intracellular and secreted proteins. Secretory PLA2 s are small (14 kDa), homologous proteins that require millimolar Ca2+ for catalytic activity
Phospholipases A2 (PLA2 s) are important enzymes in signal transduction, being responsible for release of arachidonate from membrane phospholipids for the production of prostaglandins, leukotrienes, platelet-activating factors
Phospholipase A2 Purification and Characterization: A Case Study
2 from chicken pancreatic (ChPLA2-IB) and chicken intestinal (ChPLA2-IIA) origins. Interestingly, ChPLA2-IB hydrolyzes efficiently both purified PC and PE, whereas ChPLA2-IIA hydrolyzes only PE and not PC, even after a long incubation period. These analytical
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