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文献和实验Cellular siRNA Delivery Using TatU1A and Photo-Induced RNA Interference
-permeable RNA-binding protein (RBP) by fusing the U1A RBP and a HIV-1 Tat peptide, which was labeled with an Alexa Fluor 546 fluorophore (TatU1A-Alexa). TatU1A-Alexa bound specifically to shRNA, which contains a U1A-binding sequence. The TatU1A-Alexa/shRNA
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking You want a final working concentration of 5% Serum. Order the serum from the same company and organism from which you buy secondary antibodies, Jackson Immunolabs
Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
are 5?HT1A , 5?HT1B , 5?HT2A , and 5?HT2C . In addition, methodology for using quantitative autoradiography to measure radioligand binding to serotonin receptors in brain slices is described. Protocols are provided for characterization of both saturation
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