N-Arachidonoyl-γ-Aminobutyric

Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer

Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij

Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer

% ammonium persulfate 30 microliters TEMED     pH 8.3 with acetic acid at room temp. Adjust volume to 500 ml. Running buffer is 1X TGOE buffer [Note that there is no Mg or EDTA in both the gel and in the running buffer.]

Application of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the Striatum

, TaqMan has been used to profile mRNA distribution patterns across the brain for γ-aminobutyric acid-B (GABAB ) receptor subunits (2 ), 5-hydroxytryptamine4 (5-HT4 ) receptor splice variants (3 ), novel G-protein-coupled receptors (4 ), and ion channels