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文献和实验,with the development of ESI-MS and MALDI-MS.Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases,to find proteins that show a similar theoretical digest pattern (see eg .,Shevchenko et al.1996a and b
In-gel digestion of proteins for peptide fingerprint mapping
and more sensitive alternative, with the development of ESI-MS and MALDI-MS. Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases, to find proteins that show a similar theoretical digest pattern
Utilizing Peptide SPOT Arrays to Identify Protein Interactions
of synthesized peptides 12? to 18?amino acids long, with overlapping sequences that cover the entire sequence of a protein, covalently linked to a solid support. This unit describes how to construct peptide SPOT arrays, biotinylate recombinant proteins
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