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文献和实验Purification Of Anti Peptide Antibody
the antibody can be concentrated by sweating the dialysis bags, or by spin-concentrating. Bring the azide concentration up to 0.1 and store at 4 deg C for up to three months. For longer storage freeze in aliquots and store at -80 degC or add glycerol
Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
Figure 13.6.3 Glucocorticoid receptor [F602S] LBD binding to the coactivator peptide PGC‐1 alpha (130‐154) (biotin‐DGTPPPQEAEEPSLLKKLLLAPANT‐CONH2) (A ) or the corepressor peptide NCoR (2251‐2275) (biotin
Purification Of Anti Peptide Antibody
Purification Of Anti Peptide Antibody Claire Walczak January 1996 Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls
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