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文献和实验Gene Therapy of X-Linked Severe Combined Immunodeficiency
to transduce ex vivo the CD34+cells of patients. This gene therapy protocol is currently being applied and the encouraging preliminary results published (1 ). The success of this protocol could be mainly attributed to the physiopathology of the SCID-X1 disease
Utilizing Peptide SPOT Arrays to Identify Protein Interactions
of synthesized peptides 12? to 18?amino acids long, with overlapping sequences that cover the entire sequence of a protein, covalently linked to a solid support. This unit describes how to construct peptide SPOT arrays, biotinylate recombinant proteins
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
without the need for any special equipment, and therefore can be employed in any chemical or biochemical laboratory. OBOC peptide libraries have been widely used to map the ligand specificity of proteins, to determine the substrate specificity of enzymes, and to develop inhibitors
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