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文献和实验Expression of Recombinant Membrane-Type MMPs
Since the discovery of the membrane-type matrix metalloproteinases (MT-MMPs) which are characterized by the existence of a C-terminal membrane-spanning domain followed by a short cytoplasmic “tail
Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer
blotting and employed this method to investigate cleavages generated by recombinant human Dicer in the synthetic miRNA precursors. We paid special care to visualize clearly the cleavages generated by the individual RNase III domains of Dicer
Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae
is on expression of recombinant human integral MPs in a functional state at the plasma membrane of the yeast S. cerevisiae . Optimization of yeast culture and of MP preparations is detailed for two human receptors of the Hedgehog pathway: Patched and Smoothened.
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