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100 ug
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
. Zhang, et al. (2009). "Stimulation of guanylyl cyclase-D by bicarbonate." Biochemistry 48(20): 4417-4422. 3. Long, Y., Q. Li, et al. (2011). "Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish
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