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文献和实验a DNA-microarray-based technology (Illumina) for the multi-parallel expression analysis of all known human microRNAs (http://microrna.sanger.ac.uk/; Release 10.0: August 2007). This method allowed us to process total RNA as template, allowing
of ProLong kit according to manufacturer's specifications (Molecular Probes) or use an equivalent method. About 25 µl of medium is needed per cover slip.Steps 1. Hybridization is tested before color-coding and multiple transcript detection. We started
, and synthesized on an Applied Biosystems automated DNA/RNA synthesizer (Model 392/394). Solid-support synthesis columns were from Applied Biosystems (dA, Cat. No. 400949; dC, Cat. No. 400950; dmf-dG, Cat. No. 401184; T, Cat. No. 400952). Phosphoramidites (dA, Cat
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