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北京泽平
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货号:T_70111-815-10C
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英文名称:JAR W/M PP 4OZ/125ML 12/PK
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文献和实验BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
horse anti-mouse IgG) 1/250, 30' (x1). Wash (x3) 11) 100 µL Avidin + Biotin-HRP mix (1/125 v/v Sol.A, 1/125 Sol B), 30' (x1). Wash (x3) In Coplin jars (25 mL): 12) 50 mM Tris pH8 37°C (x1)
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
in 20 mL of DMEM to be counted using a hemocytometer, and place the cells on ice. 12) Place 50 μl of the cells into a Trypan Blue solution (75 μl Trypan Blue, 125 μl PBS), mix well before placing 10 μl in the hemocytometer
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