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999
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北京泽平
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货号：T_70119-078-749
中文名称：
英文名称：XPERT CLEAR POLY 1 X 30 CONT
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
to diffuse into the construct. In addition, all of the remaining steps are conducted at room temperature. 5) Add 0.1% Triton-X in PBS to the samples for 30 minutes to permeabilize the cell membranes. 6) Rinse the samples with 3x 10
tube and remove 5-10μl lysate for protein assay. Discard pellet or, if desired, resuspend in 50-200μl of 1X sample buffer, sonicate and boil. 5) If desired/necessary, pre-clear the lysate by adding 30μl protein A-, protein-G, or protein A/G-sepharose
in perfusion fixation ** Michel's fluid for transporting cells or specimens ** Microwave ovens: Advice for new users ** Paraformaldehyde: why won't it dissolve? ** Saccomano's fixative ** Zinc-containing fixatives: What has been published? ** Alternatives
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