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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701ICN808274
中文名称:
英文名称:COOMASSIE BRILLIANT BLU 10G
货期:热销品现货,其他请咨询




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文献和实验staining your gel. The stain is: 1 g Coomassie brilliant blue R-250 50 ml glacial acetic acid 500 ml MeOH 450 ml H20 (Note: This is a somewhat weak stain recipe: it destains faster and gives a prettier gel, but may be less sensitive
in a buffer compatible with the intended next step (e.g. rehydration buffer for IEF, 0.1M Tris base + 0.1% SDS for protein assay, etc). Coomassie Staining of Gels for Mass Spectrometry 1) Fix gel for 20min in 10% acetic acid/ 25% isopropanol. Wash
with LLVY‐AMC as the substrate (left) or for Coomassie Brilliant Blue staining (middle, CBB) on a 3.6% native gel. SDS‐PAGE analysis of 7 µg of purified proteasome is shown (right, CBB). (B ) SDS‐PAGE analysis and CBB staining of purified Ub thiol esters
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