Sartorius赛多利斯16251Stainless st

Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper

bath - set to 65°C 实验步骤   1. Place the buccal swab or paper contains dried blood spot in a 2 ml centrifuge tube and add 500 ul RS1 Buffer in the tube. If RNA-free DNA is required for downstream application, add 4 ul RNase A (25

Hybridization of High Density Arrayed BAC Nylon Filter Blots

per sample well. Mixture (each sample) 2.5µl 10X reaction buffer (Perkin Elmer) 2.5µl MgCl2 (25mM) 2.0µl dNTPs (2.5mM) 1.0µl vector primer 1 (5µM) 1.0µl vector primer 2 (5µM) 0.5µl AmpliTaq (Perkin Elmer) 14.5µl ddH2 O Run

干法蛋白转膜

of the Unit for Standard Transfers (Wear gloves for this procedure to avoid contamination of membranes.)1. Remove the safety cover and the stainless steel cathode assembly.2. Place the pre-soaked sheet of filter paper onto the platinum anode. Roll a pipet