Sartorius赛多利斯16254Stainless st

Preparation of Embryonic Extract

. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml

Preparation of Embryonic Extract

. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter.      15. Cameo 0.22 um filters (Fisher Scientific).      16. 50 ml syringe for applying pressure.      17. 3x 25 ml sterile flasks.      18. 15 ml conical disposable centrifuge tubes

Preparation of Nested Deletions

solutions to be used in steps 7) - 9), including prehybridization and hybridization solutions which should be placed in a 42℃ incubator to warm. Label first 137 mm filter (Stratagene #420107) with fine tip indelible marker to correspond to plate. Holding