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16254Stainless steel filter holder,47 mm
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文献和实验Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
Preparation of Embryonic Extract
. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml conical disposable centrifuge tubes
Preparation of Nested Deletions
solutions to be used in steps 7) - 9), including prehybridization and hybridization solutions which should be placed in a 42℃ incubator to warm. Label first 137 mm filter (Stratagene #420107) with fine tip indelible marker to correspond to plate. Holding
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