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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311705758
中文名称：
英文名称：X1000 TIES NYLON BLUE 20X5,4CM
货期：热销品现货，其他请咨询
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文献和实验SOUTHERN BLOTTING OF GENOMIC DNA
of 5 volts per cm. • run until the bromophenol blue has run ca.9 cm (some DNA will run 1-2 cm below the dye). • Using gloves,place in staining solution (0.5 to 1 μg/ml ethidium bromide in water,20-40 min.Be careful not to break the gel. 3.Nicking of DNA
. 20X SSC Stock Solution Add 175.3g NaCl and 88.2g sodium citrate to 800 ml distilled water and pH to 7.0. Add water to 1000 ml and autoclave to sterilize. Removing Probe From Nylon Membranes 1. If reprobing is desired, do not allow the Zeta-Probe
the RNA to run into the gel first so that the buffer circulation won't disturb it in the wells. It takes 2.5 hours to run the gel about 11 cm; the gel and buffer will become warm during the run. 6. After the gel is run, no further treatment of the gel
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