赛默飞世尔Thermo Fisher ASTM WATER

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

DNA Solutions （DNA分离）

Tris-HCl, 20 mM EDTA. Diatomaceous earth-wash buffer : 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, pH 8.0, 50% ethanol in double distilled water. 10 ml 1 M Tris-HCl, pH 8.0 2 ml 0.5 M EDTA, pH 8.0 500 ml 100% ethanol (McCormick Distilling Co., Inc.) ddH