赛默飞世尔Thermo Fisher MF75 消毒过滤器

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

of CTO stock solution into 18 ml of serum-free MCDB-131 medium.     4)      Rinse EPCs in 3 near confluent T-75 cell culture flasks with 10 ml DPBS (without Ca or Mg).     5)      Add 6 ml of the 2 μM CTO working solution to each flask

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

Southern Hybridization Protocols

-0.1% SDS —> 250ml kept at 65 ℃ W1 = 0.1SSC -0.1% SDS —> 250ml kept at 65 ℃W1W2W315ml 20XSSC6.25ml 20XSSC1.25ml 20XSSC3.75ml 20% SDS1.25ml 20% SDS1.25ml 20% SDS131.25ml H2O242.5ml H2O247.5ml H2O150.00ml250.00ml250.00mlDrain the solution