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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 保修期:
两年
- 现货状态:
现货
- 供应商:
科邦兴业(北京)科技有限公司
- 规格:
盒
13440-130------KGFpre-filter;binder free;130mm;50pc
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文献和实验, 1.4 numerical aperture oil objective and a CCD camera with a 1,317 × 1,035 Kodak chip (pixel size 6.8 μm × 6.8 μm; Princeton Instruments Inc.). Blue fluorescence is detected using the excitation filter 350/50 D, the beamsplitter 400 DCLP and the emission filter
Recycling Tubulin We "recycle" tubulin fractions stored at -80¡C after the PC column and store the recycled tubulin in small aliquots for day-to-day use. We generally store recycled tubulin in Injection Buffer (IB) without free GTP
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
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