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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 保修期:
两年
- 现货状态:
现货
- 供应商:
科邦兴业(北京)科技有限公司
- 规格:
盒
VS0121VIVASPIN 500 30,000 MCWO PES 25/BOX
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文献和实验PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction: 1 x 95 °C, 2 min 21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min 1 x 72
In Situ Hybridization Using Digoxigenin Labeled Probes
morphology. After the pretreatment the slides should be hybridized within 24 hours. 1) Place the slides in 2X SSC at 65°C for 30 minutes 2) Wash for 2 minutes in 2X SSC at room temperature. 3) Acetylate the slides as follows: Prepare 500 ml of 0.1 M
(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
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