Sartorius赛多利斯VS0121VIVASPIN 50

Chromatin IP (CHIP assay)

PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction:      1 x 95 °C, 2 min      21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min      1 x 72

In Situ Hybridization Using Digoxigenin Labeled Probes

morphology. After the pretreatment the slides should be hybridized within 24 hours. 1) Place the slides in 2X SSC at 65°C for 30 minutes 2) Wash for 2 minutes in 2X SSC at room temperature. 3) Acetylate the slides as follows: Prepare 500 ml of 0.1 M

人单核细胞和中性粒细胞

(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum