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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 保修期:
两年
- 现货状态:
现货
- 供应商:
科邦兴业(北京)科技有限公司
- 规格:
盒
13440-100------KGFpre-filter;binder free;100mm;50pc
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文献和实验. Remove the supernatant and resuspend the hexokinase pellet in 5 μl glucose solution (250 mM). Cytosol mix (volumes correspond to one reaction of 50 μl; scale up accordingly): 100 μg cytosol (usually 10�17 μl, depending
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
Small Dounce (2 ml) 50.2Ti rotor (warm = 37¡C) TLA100.2 or 100.3 rotor (cold = 2-4¡C) II. Recycling Protocol 1. Thaw 3-4 3 ml PC column fractions at 37¡C. Transfer to ice and mix in a 50 ml conical. 2. Add BRB80
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