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VS15RH21VIVASPIN 15R 30,000 MWCO HYDRO 12/BOX
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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
4 2 x 106 EP + DNA Normal % survival after EP + DNA 5 2 x 106 EP + DNA G418 % total integrants ie random + H/R's
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
(2 x 106 cells ) + 0.73 ml PBS To A add 15 ug linearised plasmid DNA, IMMEDIATELY prior to electroporation.. B no DNA 3. Using a Biorad Gene Pulser, (McMahon, A.P., and Bradley, A. (1990) Cell 62 1073-1085). "Zap" each curvette twice i.e
In Situ Hybridization Using Digoxigenin Labeled Probes
move to the opposite edge. Often better results are obtained by lightly streaking the slide several times, rather than streaking hard once or twice 12) Blot off excess fixative and examine under the microscope using phase contrast. If spreading
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