Sartorius赛多利斯13400-120------KG

Random Primer DNA labeling

-----------  = dpm/µg (ng DNA synth. + ng input DNA) x 0.001µg/ng Note The factors 20 and 50 are derived while using 5µl of 1:100 dilution for DE-81 filter-binding assay

PC12 Cell Culture and Fusion

1. Nerve growth factor (2.5S NGF, Bioproducts for Science). Prepare 1 mg/ml stock in 50 mM NaAc, pH 5.0. Sterile filter and store as 5 ul aliquots in 15 ml conical tubes at -80o C. 2. NGF medium: Add 1 ml of F12K medium to each tube of NGF aliquot

Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage

:   Freezing media: (1 liter) Prepare a 1 liter volume and divide into 25-50 ml centrifuge tubes containing 40 ml each. Store the tubes at -80 degrees C for up to one year. 700 ml RPMI-1640 with 2mM L-Glutamine 200 ml