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- Sartorius
- 11106-142------N
- 德国
- 2025年11月09日
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11106-142------NCA Membrane_ 0.45µm_ 142mm_ 100pk
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文献和实验Preparation of Brain Membrane Fractions
in approximately 5 ml water. Add sucrose and water to give a final volume of 45 ml 1.2 M sucrose, add HEPES to 5 mM and the protease inhibitors as above. Homogenize in glass FEP polymer-homogenizer (3 strokes, 1000 rpm).Spin at 9200 x g for 20 min.Resuspend pellets
Membrane Transfer and Crosslinking for RNA
The composition of the transfer buffer is usually a 5X SSC/10 mM NaOH solution. These mildly alkaline conditions shear the RNA into smaller fragments and denature it as it is deposited onto the membrane. A brief protocol for assembly (see Figure 1
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
reaction containing 10mM Tris-Hcl (pH 8.3), 50 mM KCl, 2 mM MgCl2 , 50µM dNTP, 0.5µM each primer, and 0.6 unit of Taq DNA polymerase (Cinagen, Tehran, IRAN). The primers used were 3-A (5'-TAGTCACTGGCAACAGTCTT-3') and 3-B (5'-AAAATCCCTGTTCCCACTCATAC¨C3
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