Sartorius赛多利斯14459--25------DR

【交流】关于NEB的T4 DNA连接酶和快速连接试剂盒的常见问题及解答

(4)。 一个韦氏单位=67个粘端连接活性单位。 浓度：400,000 units/ml和2,000,000 units/ml。 贮存条件： 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 200 µg/ml BSA和50%的甘油，-20°C贮存。 热失活条件: 65°C 加热10分钟。 室温连接反应：为方便起见，连接反应可以在室温（20-25°C）条件下进行。粘性末端连接：在20µl反应体系中加入1µl T4 DNA

体外荧光法检测核内体早期动力学

from Isobiotech. Critical Clearance has to be 20 μm for PC12 cells and may be different for other cells, e.g., 25 μm for BHK cells. Open imaging chamber for 18-mm coverslips For imaging, we use a chamber

Preparation of Carboxypeptidase Y and Its Properties

℃ for 1 hr. In the presence of 10% methanol, the enzyme is completely stable at pH 5.5～8.0 for 8 hr at 25℃. Aqueous solu­tion of the enzyme is stable for 8 hr in the pH range 5.5～8.0 at 25℃ and for 2 hr in the pH range 6～8 at 37℃. At higher temperatures