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文献和实验Production of a Human Antibody Library in the Phage-Display Vector pSEX81
(1 ,2 ). A promising alternative 1s the production of human recombinant antibodies (3 ). Recombinant DNA technology has made it possible to clone human antibody genes in vectors and to generate antibody expression libraries (4–7). One approach has been to amplify
Construction and Manipulation of Large-Insert Bacterial Clone Libraries
Introduction Construction of Large-Insert Bacterial Clone Libraries Fig. 1. A general procedure
Human Antibodies by Guided Selection
. This chapter will describe the sequential guided selection procedure using a Fab format as an example. The first step is to clone the variable regions of a mouse antibody to a phagemid vector containing human constant domains, C? and CH1, which results
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