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IL-1α/IL1F1,Human,Recombinant

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  • ¥1780 - 35580
  • 普诺赛
  • PCK045
  • 国产
  • 2025年07月30日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      现货

    • 供应商

      上海经科化学科技有限公司

    • 规格

      10μg/500μg/50μg/1mg

    规格:10μg产品价格:¥1780.0
    规格:500μg产品价格:¥24080.0
    规格:50μg产品价格:¥4980.0
    规格:1mg产品价格:¥35580.0

    产品信息

    别名 Hematopoietin-1, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte
    物种 Human
    表达宿主 E.coli
    序列信息 MSAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA with polyhistidine tag at the C-terminus.
    检索号 P01583.1
    分子量 18.99 kDa
    表观分子量 17 kDa
    标签 His-tag at the C-terminus
    生物活性 Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is <10 pg/mL. The specific activity of recombinant human IL-1 alpha is approximately >1 x10^8 IU/ mg.

    产品特性

    纯度 >98% as determined by SDS-PAGE. Ni-NTA chromatography
    内毒素 <0.1 EU per 1 μg of the protein by the LAL method.
    保存 Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution,store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
    运输 Ambient temperature or ice pack.
    制剂 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
    复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

    背景介绍

    Interleukin 1 alpha (IL-1α) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.

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    图标文献和实验
    相关实验
    • Detection of autoantibodies to cytokines

      Autoantibodies to various cytokines have been reported in normal individuals and in patients with various infectious and immunoinflammatory disorders, and similar antibodies (Ab) may be induced in patients receiving human recombinant cytokines

    •  神经免疫内分泌调节环路

      有GC限制IL-1的进一步生成,且ACTH前体POMC还可裂解释放α-MSH,而α-MSH可在中枢水平对抗IL-1对CRH分泌的刺激效应。见图10-5。 (二)下丘脑-垂体前叶-肾上腺皮质与胸膛环路 此环路有如下环节: (1)HPA轴中ACTH和GC均可抑制胸腺的功能,包括细胞增殖及胸膛激素分泌。 (2)胸腺激素中thymosin α1及thymulin等都能刺激ACTH的分泌。 (3)胸腺中含CRH受体并可合成CRH,而CRH对胸腺的某些功能有刺激

    • 核酸标记技术检测法

      切片进行原位杂交,然后进行放射自显影。 3.细胞因子mRNA经反转录为cDNA,用特异性细胞因子引物经聚合酶链反应(PCR)扩增细胞因子cDNA,Southern blot后用标记探针检测特异细胞因子DNA水平。 (金伯泉) 参 考 文 献 1.Ared WP,et al.An IL-1 inhibitor from human monocytes production and characterization of biologic properties.J

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