- ￥3870 - 8940
- Sino Biological
- 北京
- 10620-H41H-B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human BCMA Protein (ECD, Fc & AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg
| 规格： | 20 µg | 产品价格： | ￥3870.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥8940.0 |
BCMA重组蛋白|Recombinant Human BCMA Protein (ECD, Fc & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human BCMA Protein (ECD, Fc & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human TNFRSF17 (NP_001183.2)(Met1-Ala54) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：1.Immobilized Recombinant Human BLyS / TNFSF13B / BAFF Protein (Fc Tag) ( Cat:10056-H01H) at 2μg/mL (100μL/well) can bind Recombinant Human BCMA Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 10620-H41H-B), the EC50 is 0.8-2.5 ng/mL. 2.Immobilized Anti-BCMA Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human BCMA Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 10620-H41H-B), the EC50 is 0.6-4 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 1
蛋白分子量：The recombinant human TNFRSF17 consists of 237 amino acids and predicts a molecular mass of 27.80 kDa. It migrates as an approximately 41.44 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001183.2
蛋白氨基酸序列：Met1-Ala54
蛋白标签：C-human IgG1-Fc & AVI
蛋白名称：Human BCMA Protein (ECD, Fc & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human TNFRSF17 (NP_001183.2)(Met1-Ala54) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：1.Immobilized Recombinant Human BLyS / TNFSF13B / BAFF Protein (Fc Tag) ( Cat:10056-H01H) at 2μg/mL (100μL/well) can bind Recombinant Human BCMA Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 10620-H41H-B), the EC50 is 0.8-2.5 ng/mL. 2.Immobilized Anti-BCMA Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human BCMA Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 10620-H41H-B), the EC50 is 0.6-4 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 1
蛋白分子量：The recombinant human TNFRSF17 consists of 237 amino acids and predicts a molecular mass of 27.80 kDa. It migrates as an approximately 41.44 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001183.2
蛋白氨基酸序列：Met1-Ala54
蛋白标签：C-human IgG1-Fc & AVI
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文献和实验相关实验
处理过的融合蛋白的2倍以上，这也说明标签的存在降低了该酶的活性。可以用固化了Cu2+的亲和层析柱去除切除的融合标签。 ■ 八种人趋化因子（Magis-trelli 2005）——所有的蛋白都在OrigamiTM B菌株中表达提高它们在胞质中的二硫键形成率。在趋化因子编码序列的C端引入了AviTMTag（亲和素）生物素化序列。切割后的细胞趋化因子可以用单体的亲和素树脂亲和层析与切割下的NusA标签和蛋白酶混合物分离开。所有切割纯化后的蛋白在细胞趋化实验中都显示了活性，而没有一个融合蛋白有这样的活性
的存在降低了该酶的活性。可以用固化了Cu2+的亲和层析柱去除切除的融合标签。 ■ 八种人趋化因子(Magis-trelli 2005)——所有的蛋白都在OrigamiTM B菌株中表达提高它们在胞质中的二硫键形成率。在趋化因子编码序列的C端引入了AviTMTag(亲和素)生物素化序列。切割后的细胞趋化因子可以用单体的亲和素树脂亲和层析与切割下的NusA标签和蛋白酶混合物分离开。所有切割纯化后的蛋白在细胞趋化实验中都显示了活性，而没有一个融合蛋白有这样的活性。 ■ 蚯蚓血红蛋白(Karlsen
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Sino Biological BCMA重组蛋白 ECD Fc AVI标签 生物素化 bcma protein
￥3870 - 8940
