Sartorius赛多利斯17764--------ACK针

类器官培养与应用——血管类器官

基，放置在 37℃，5％CO2 培养箱中进行培养。   诱导中胚层分化 7、hPSCs 培养 1 天至形成具有平滑边界的聚集体，直径 >50-100 µm 。 8、聚集体形成后，将所有细胞转移至 15ml 离心管中，依靠重力自然沉降（注意：不建议将聚集体离心处理）。静置 15-20min 后，小心吸去培养基上清。 9、将聚集体用 10ml hPSCs 中胚层分化培养基轻轻重悬，加入到低吸附的 6 孔板中，每孔 3ml，放置在 37℃，5％CO2 培养箱中孵育 3 天以诱导中胚层分化，培养期间每天

Purification of TFIIIC

and additions: 1.To make saturated culture combine 5 ml of YPD with 25µl of 0.4% adenine and 1 blob of yeast.Incubate at 30℃ for 18 to 24 hr until yeast is at a density of 2.0 x108 cells/ml. 2.Grow cells to an OD600 of ~1.0 after inoculating 1 liter of YPD

Purification of TFIIIC from yeast

and additions: 1.To make saturated culture combine 5 ml of YPD with 25µl of 0.4% adenine and 1 blob of yeast.Incubate at 30℃ for 18 to 24 hr until yeast is at a density of 2.0 x108 cells/ml. 2.Grow cells to an OD600 of ~1.0 after inoculating 1 liter of YPD