Sartorius赛多利斯11105-142------GC

常用试剂配制

/M 55.03g EDTA, pH 8.0 2mM 20mM 500mM 40ml 43.84g H2 O     mQ 863.3ml 10x stock solution is stable at NT after 0.4µm filtration; for PAAG use as 1x, for agarose gel - as 0.5x; TBE is used in agarose gels

A/B/C Random Amplification Protocol

DNA. Round B PCR   30 µL  Round A DNA product   20 µL 10X Thermo Pol PCR Buffer     2 µL 25 mM dNTP     4 µL  100 µM Primer B Cy5 (sample) or Cy3 (reference DNA)     2 µL  Ampli Taq  0.1 µL Pfu Turbo 142 µL dH 2 O    

Southern blot实验安排总结

1mM EDTA 0.5M：100µl 鲑鱼精DNA 100µg/µl 10mg/ml：50µl ddH2O溶解至45ml，与5ml 1%BSA 混合，定容至50ml水中。 洗膜液I(磷酸系统) 100ml BSA 0.5% 0.5g EDTA 1mM 0.5M:200µl NaHPO4(PH7.2) 40mM 1M:4ml SDS 5% 20%:25ml 洗膜液II(磷酸系统) 100ml EDTA 1mM 0.5M:200µl NaHPO4(PH