Sartorius赛多利斯18406-100------GR

Single Primer ("Semi-Random") P

Reamplification 40 µL H2O 10 µL 10x M-PCRB 10 µL 4 dNTPs @ 2 mM each 10 µL Pn primer @ 5 µM 10 µL Po primer @ 0.2 µM 10 µL DNA from part 1 10 µL "T/TS" @ 0.4 u Taq/µL --------- 100 µL Load glass capillaries and amplify: 30"x94°C 30

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

peptides is also described.MATERIALSReagentsAcetic acid (100 mM)Ammonium bicarbonate (50 mM, pH 8.0)Calf intestinal alkaline phosphataseCarboxypeptidase Y (1 µg/µL in H2O)EDTA (100 mM in H2O, pH 8.0)Ferric chloride (FeCl3) (100 mM)Gallium chloride (GaCl

基于PCR技术的染色质沉淀分析

µCi/µl, specific activity 3000 Ci/mmol) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)dNTPs (stock solutions at 25 mM for each dNTP)DNA loading buffer (6X)30% (v/v) glycerol0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FFStore