赛默飞世尔Thermo Fisher PIPET SHORT

In Situ HYBRIDIZATION TO TISSUE SECTIONS

2O. Pronase treat: -Remove the slides from the rack and place horizontally in a tray. -With a Pasteur pipet, carefully drip ‰0.25mg/ml pronase solution onto the sections and incubate 10' (thhe incubation time starts with treatment

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

each cycle(c) 25 more cycles of ●● 94°C for 15 s ●● 64°C for 30 s ●● 70°C for 2 min 10 s (d) Final extension at 70°C for 10 min (e) 4°C hold 10. Check secondary PCR results by resolving 10 mL of eachsample in 1% agarose gel