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- 文献和实验
- 技术资料
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999
- 供应商：
北京泽平
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1年
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EA
货号：T_701M64000WB94
中文名称：
英文名称：MIDAS III WATER BATH
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文献和实验Southern Hybridization Protocols
distilled water or TE in a microcentrifuge tube by heating for 5 min in a boiling water bath; then immediately cooled on ice. Perform the following adddtions on ice: 1 µ l 500 µ M dATP 1 µ l 500 µ M dGTP 1 µ l 500 µ M dTTP 20 µ l 2.5x Random
for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
solution ( 36.5%; Sigma-Aldrich 33220) Glycine (2 M; Fisher BP381) Glycogen (Roche) H2 O, nuclease-free Immune complex wash buffers (high-salt and low-salt) Prepare the high-salt and low-salt versions of this buffer
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