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文献和实验Chromatin Assembly Using Drosophila Systems
Figure 21.7.1 Chromatin assembly by the S‐190 extract. Lanes 1, 2—complete system, as described in ; lanes 3, 4—no S‐190 extract; lanes 5, 6—no core histones (note residual assembly activity with the endogenous histones of the extract); lanes 7, 8—
RAMPAGE: Promoter Activity Profiling by Paired‐End Sequencing of 5′‐Complete cDNAs
) 1 M Tris‐Cl, pH 7.0 and 8.5: prepare from commercial pH 7.4 stock (Sigma‐Aldrich, cat. no. T2194–100ML) by adjusting pH with HCl or NaOH Biotin hydrazide, long arm
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
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