赛默飞世尔Thermo Fisher 24 DBLE-DEE

SDS-PAGE

. 0.1% SDS. Keep a squirt bottle of it.VIII. Running buffer: Keep a jug of this. 24 g Tris base, 115.2 g glycine, 20 ml 20% SDS, H2O to 4 liters.IX. Sample buffer: 100 mM Tris, pH 6.8, 2% SDS, 5% ß- mercaptoethanol, 15% glycerol, enough bromophenol blue

Technovit® 9100 Methyl Methacrylate

according to Schaffer (Formol/Alcohol). 1.4% paraldehyde solution at 4-8°C for 24-48 h. (see table for sensitive detection of enzymes such as alkaline phosphatase and for antigens sensitive to denaturation /structural changes.) Dehydration, Intermediate

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

?cyanoethylthio group is effected by treatment with 2,4,6?triisopropybenzenesulfonyl chloride and triethylamine to give a 6?(2,4,6?triisopropylbenzenesulfonic acid) ester intermediate. Reaction of this key intermediate with 3?mercaptoproprionitrile