赛默飞世尔Thermo Fisher Kapillarsäu

RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes

tablet (#B5655) ; Dissolve one tablet in 10 ml staining solution. If there is a non-specific background signal, you can dilute more (up to 20 ml).       Culture tubes : Available from Fisher as 6 mm (ID) x 50 mm (length

In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes

or into 6 mm x 50 mm culture tubes). 5) Using a 10 mg/ml stock of Proteinase K (PrK), make solutions of 25 ug/ml and 40 ug/ml PrK in PBTw. Divide the gonads in half. Digest one half of the gonads in 3 ml of 25 ug/ml PrK / PBTw for 30 min and the other half

Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)

(Fisher Scientific) Chemotaxis Buffer    25 mM Hepes, pH 7.4 1% (v/w) Endotoxin-free Bovine Serum Albumin prepare in RPMI 1640 medium