078664赛默飞Acclaim C30,3µm,2.1x2

Noninvasive Human Nuclear Transfer with Embryonic Stem Cells

Syringe (1-mL) (Fisher Scientific) Thermo plate (MATS) METHOD Experiment Set-Up   1. Prepare culture media (two dishes of cleavage medium with 10% SPS, and one dish of 2 mM DMAP): i. Place 10-15 drops

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

solution ( 36.5%; Sigma-Aldrich 33220) Glycine (2 M; Fisher BP381) Glycogen (Roche) H2 O, nuclease-free Immune complex wash buffers (high-salt and low-salt) Prepare the high-salt and low-salt versions of this buffer

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C