赛默飞世尔Thermo Fisher BIO TANK CL

Southern Hybridization Protocols

tube itself. Preparations of washing solutions: 2xSSC/0.1%SDS: 5 ml 20xSSC, 0.25 ml 20%SDS, 44.75 ml distilled water. 1xSSC/0.1%SDS: 2.5 ml 20xSSC, 0.25 ml 20%SDS, 47.25 ml distilled water. Autoradiography: After final wash, remove membrane

Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applicati

(Fisher Scientific) 1.5‐ml screw‐cap polypropylene centrifuge tubes 55°C and 65°C heating

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

11.33 ml 11.33 ml Gel A/B 5.37 ml 6.90 ml 8.44 ml 9.20 ml 9.97 ml 11.50 ml Gel A 583 µl 750 µl 916 µl 999 µl 1.08 ml 1.25 ml TEMED 14.84 µl 13.6 µl 12.36 µl 11.75 µl 11.13 µl 9.89 µl 10% APS 148.4 µl 136 µl 123.6 µl 117.5 µl 111.3 µl 98.9 µl