赛默飞世尔Thermo Fisher CASTING TRA

2-D Polyacrylamide Gel Electrophoresis

-Gel casting Chamber (Bio-Rad,#165-2025)as follows: Bottom of chamber Small plate,20 cm Spacers,20 cm x 1 mm Eared plates,20 cm Spacers,20 cm x 1 mm Large plate,20 cm Meylar sheet Repeat as needed. 5.Fill the chamber with acrylic blocks

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

for counting. Live cells are clear while dead cells are blue. Expect approximately 3 million cells per rat pup, with a viability of approximately 80-90%. 5. Casting fibrin gel constructs - preparation for creating fibrin gels (done well in advance)

重组DNA的分离、克隆与测序实验

casting, dried agarose is dissolved in buffer by heating and the warm gel solution then is poured into a mold (made by wrapping clear tape around and extending above the edges of an 18 cm X 18 cm glass plate), which is fitted with a well-forming comb