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- 库存:
999
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北京泽平
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货号:T_70112-565-348
中文名称:Nunc EasYFlask无菌培养瓶
英文名称:FLSK 25CM2 VNTCLOS 7ML 200/CS
货期:热销品现货,其他请咨询




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文献和实验in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
). The only apparent problem with removing the cross braces is that now they're slightly imbalanced (and they make me nervous). Nunc Omnitrays: Rectangular petri dish, handy for using 12 channel pipettor to plate. (Fisher #12-565-296; $34/60). These can be washed
ES / MEF cell culture and electroporation of targeting construct
with 70% ethanol and transfer the contents of the vial into one 75 cm2 flask (T-75) containing 20 ml of MEF media. Place the MEFs in a 37o C, 5% CO2 , 86% humidity incubator. Every frozen MEF preparation thaws a little differently. If on Day 1, the MEFs
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